The Product
mCRP-ALISA™ — a quantitative readout for monomeric CRP
A microplate assay built to fit the workflows labs already run, engineered around a binder that sees the active form of CRP.
At a glance
What's in the kit
| Component | Specification |
|---|---|
| mCRP-coated microplate | 96-well, COOH-activated; mCRP immobilised |
| Biotinylated Sentos binder | Working range ~0.3–4.8 µg/mL; specific for mCRP (KD ≈ 151 nM) |
| Detection system | Streptavidin-HRP conjugate; TMB substrate; acid stop solution |
| Buffers & solutions | Aptamer binding buffer; PBS-Tween wash; blocking solution |
| Quality controls | Positive control (recombinant mCRP, 100 ng/mL); negative control (pooled normal serum); multi-point reference standards |
Workflow
Designed for the lab bench
A single-plate, colorimetric workflow that maps onto standard ELISA-style processing — no specialised reader beyond a standard microplate spectrophotometer. TODO: final assay time target for public claim
- 1
Prepare
thaw reagents; lay out standards, controls, and samples.
- 2
Incubate
competitive binding of sample and plate-bound mCRP for the aptamer.
- 3
Wash & develop
remove unbound material; add streptavidin-HRP and TMB.
- 4
Read
colorimetric measurement; interpolate mCRP from the standard curve.
Performance & specifications
Specifications (development-stage)
| Parameter | Value |
|---|---|
| Target analyte | Monomeric C-reactive protein (mCRP) |
| Sample type | Human serum |
| Limit of detection | Approximately 100 ng/mL in serum |
| Specificity | >95% discrimination vs pCRP |
| Cross-reactivity | <5% vs pCRP; <1% vs common serum proteins |
| Coated-plate stability | 12 weeks at 2–8 °C (validated) |
| Extended-stability target | 18-month stabilised shelf life — development target under evaluation TODO: Not yet validated |
| Format | 96-well microplate, competitive ALISA, colorimetric |
Regulatory status
Why it's different
Three advantages that compound
A specific target.
Most inflammation tests measure total/pentameric CRP. Ours is built to measure the monomeric, tissue-active form.
Aptamer chemistry.
Synthetic, reproducible, and robust — with a favourable cost structure at scale versus antibody-based assays.
A workflow labs already know.
A microplate competitive immunoassay that slots into existing lab operations.
Roadmap (product formats)
From microplate to point of care
The microplate assay is the first format. Our development roadmap includes a point-of-care configuration and multiplex panels that pair mCRP with complementary inflammatory markers. These are in development and not yet available. TODO: which roadmap items are public